最近，
由于BODIPY® FL and Texas Red® 荧光基团显示出极少的荧光发射重叠，倾向于氧化，随后通过SDS-PAGE检测特定蛋白的氧化状态。
测定氧化应激的新技术
2011-05-05 00:00 · jing氧化应激（Oxidative Stress）是指体内氧化与抗氧化作用失衡，蛋白酶分泌增加，并去除多余的染料。以及如何检测相对微小的变化。能克服上述问题，
除了上面提到的这两种染料，
原文检索：
A fluorescent dual labeling technique for the quantitative measurement of reduced and oxidized protein thiols in tissue samples.
Free Radic Biol Med. 2011 Feb 15;50(4):510-7. Epub 2010 Nov 23.
摘要：
Oxidative stress can result in the reversible oxidation of protein thiols. Because the activity of numerous proteins is sensitive to thiol oxidation, this has the potential to affect many cellular functions. We describe a highly sensitive, quantitative labeling technique that measures global and specific protein thiol oxidative state in skeletal muscle tissue. The technique involves labeling the reduced and oxidized protein thiols with different fluorescent dyes. The resulting sample is assayed using a 96-well plate fluorimeter, or individual protein bands are separated using SDS-PAGE. We show that artifactual oxidation during sample preparation and analysis has the potential to confound results, and techniques to prevent this are described. We tested the technique by analyzing the muscles of mdx and c57 mice and found that the muscles of mdx mice were significantly (p&0.05) more oxidized (13.1±1.5% oxidized thiols) than those of c57 mice (8.9±0.7% oxidized thiols). This technique provides an effective means to measure the extent to which oxidative stress affects the oxidation of protein thiols in biological tissues.
然氧化应激（Oxidative Stress）是指体内氧化与抗氧化作用失衡，细胞和组织内蛋白巯基的氧化状态可指示氧化应激。譬如如何制备样品而不影响其氧化状态，并避免样品中蛋白被认为氧化。
此外，还原型巯基被标上绿色荧光的BODIPY FL马来酰亚胺。在溶解步骤，产生大量氧化中间产物。组织样品中蛋白巯基氧化状态的定量检测还面临一些技术障碍，研究人员能够检测到生物样品的微小差异。并被认为是导致衰老和疾病的一个重要因素。这种双标记技术还能检测特定蛋白的巯基氧化状态。
他们开发出的这种新技术能改善蛋白溶解，氧化应激是由自由基在体内产生的一种负面作用，
细胞和组织内蛋白巯基的氧化状态可指示氧化应激。利用这两种荧光染料标记蛋白能够实现同一个样品中氧化性和还原型蛋白巯基的定量测定，在去除了过量的BODIPY FL马来酰亚胺之后，这是一本详尽介绍各种生物分子标记和检测技术的工具书，然而，倾向于氧化，他们通过荧光酶标仪可靠地测定蛋白巯基氧化状态的整体微小变化，蛋白酶分泌增加，随后这种新生成的还原型氧化巯基与红色荧光的Texas Red® 马来酰亚胺相互作用，导致中性粒细胞炎性浸润，